Posted by Personal glucose meter can be used for at home dyagnosis of tumor utilising the catalytic effect of β-cyclodextrin.
Benefiting from our discovery that β-CD could enhance the catalytic activity of invertase through hydrogen bonding to improve detection sensitivity, a highly sensitive and convenient biosensor for the detection of miR-21 was proposed, which is based on the simplicity of reading signals from a personal glucose meter (PGM), combined with self-assembled signal amplification probes and the performance of β-CD as an enhancer. In the presence of miR-21, magnetic nanoparticle coupled capture DNA (MNPs-cDNA) could capture it and then connect assist DNA/H1-invertase (aDNA/H1) and self-assembled signal amplification probes (H1/H2) in turn. As a result, a “super sandwich” structure was formed. The invertase on MNPs-cDNA could catalyze the hydrolysis of sucrose to glucose and this catalytic process could be enhanced by β-CD. The PGM signal exhibited a linear correlation with miR-21 concentration within the range of 25 pmol L−1 to 3 nmol L−1, and the detection limit was as low as 5 pmol L−1 with high specificity. Moreover, the recoveries were 103.82–124.65% and RSD was 2.59–6.43%. Furthermore, the biosensor was validated for the detection of miR-21 in serum, and the results showed that miR-21 levels in serum samples from patients with Diffuse Large B-Cell Lymphoma (DLBCL) (n = 12) were significantly higher than those from healthy controls (n = 12) (P < 0.001). Therefore, the ingenious combination of PGM-based signal reading, self-assembled signal amplification probes and β-CD as an enhancer successfully constructed a convenient, sensitive and specific biosensing method, which is expected to be applied to clinical diagnosis.
Feng Yao, Longjie Wu, Yamin Xiong, Chaojie Su, Yujing Guo, Shajidan Bulalea Miaomiao Zhou, Yongmei Tian and Leiliang He (2024) A novel β-cyclodextrin-assisted enhancement strategy for portable and sensitive detection of miR-21 in human serum. Anal. Methods, 16, 1639-1648. https://doi.org/10.1039/D3AY02269B
Picture credit to: https://e77.hu/termekek/vercukormero-rendszer/dcont-etalon