Posted by To overcome the hydrophobicity and effumability restriction of the essential oil in practice, we aim to fabricate the (E)-2-octenal-β-cyclodextrin inclusion complex (O/β-CDIC) and investigate its encapsulation mechanism.
The O/β-CDIC was fabricated using saturated aqueous solution method combined with freeze-dried method. The encapsulation efficiency and loading content of O/β-CDIC were 45.61% ± 1.37% and 2.97% ± 0.08%. In vitro assays, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the O/β-CDIC were 2.00 and 8.00 mg·mL−1 for Penicillium italicum XX5 mycelial growth, respectively. In addition, the O/β-CDIC effectively reduced the incidence of blue mold in citrus fruit and maintained fruit quality. The detailed structure characterization combined with the molecular docking study, molecular electrostatic potential, and independent gradient model based on Hirshfeld analysis collectively demonstrated that (E)-2-octenal was entrapped successfully within the β-CD cavity.
The O/β-CDIC significantly enhanced the thermal stability and phase solubility of (E)-2-octenal while effectively preserving its antifungal activity. Characterization analyses confirmed the presence of non-covalent interactions between β-CD and (E)-2-octenal.
Yuwei Luo, Yonghua Zhang, Xiumei Chen, Jinxin Che, Nengguo Tao, Preparation, characterization, and antifungal activity of (E)-2-octenal/β-cyclodextrin inclusion complex against the blue mold in citrus fruit caused by a prochloraz-resistant Penicillium italicum strain, Journal of Applied Microbiology, Volume 136, Issue 5, May 2025, lxaf115, https://doi.org/10.1093/jambio/lxaf115
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