In response to the COVID-19 and monkeypox outbreaks, we present the development of a universal disinfectant to avoid the spread of infectious viral diseases through contact with contaminated surfaces. The sanitizer, based on didecyldimethylammonium chloride (DDAC), N,N-bis(3-aminopropyl)dodecylamine (APDA) and γ-cyclodextrin (γ-CD), shows synergistic effects against non-enveloped viruses (poliovirus type 1 and murine norovirus) according to the EN 14476 standard (≥99.99% reduction of virus titer). When a disinfectant product is effective against them, it can be considered that it will be effective against all types of viruses, including enveloped viruses. Consequently, “general virucidal activity” can be claimed. Moreover, this synergistic action has been extended to bacteria (P. aeruginosa, EN 13727). The virucidal mechanism was ascribed to: (i) the protonation of APDA leading to pH modification and facilitating the co-micelles dissociation, (ii) the partial denaturation of the viral capsid proteins upon pH changes facilitating the passage of APDA and DDAC through the capsid, (iii) the charges screening of the viral nucleic acid, (iv) the full dislocation of the viral capsid, and (v) the prevention of the protein’s re-aggregation by the γ-CDs via chaperone-like activity. On the flip side, the bactericidal action was assigned to: (i) the modification of pH upon the protonation of APDA, (ii) the fast insertion/removal between the cations (DDAC and charged APDA) and the phospholipids, and (iii) the lipid extraction by the γ-CDs. This synergistic mixture could then be highly helpful as a universal disinfectant to avoid the spread of infectious viral or bacterial diseases in community settings, including COVID-19 and monkeypox (caused by enveloped viruses).
Loïc Leclercq and Véronique Nardello-Rataj (2022) A New Synergistic Strategy for Virus and Bacteria Eradication: Towards Universal Disinfectants.. Pharmaceutics 2022, 14(12), 2791; https://doi.org/10.3390/pharmaceutics14122791