Application of cyclodextrin/polyethylene glycol in blood-storage tools for liquid biopsy

Posted by

In recent years, liquid biopsy has garnered considerable attention as a valuable diagnostic technology for cancer. An analysis target of liquid biopsy is cell-free DNA; however, contamination with wild-type DNA released from injured leukocytes leads to low test accuracy. Thus, there is a need for blood-storage tools that inhibit the release of wild-type DNA from leukocytes. In the present study, we demonstrated that γ-cyclodextrin (γ-CyD) and polyethylene glycol (PEG) synergistically inhibit DNA release from cells by inhibiting cell aggregation. In addition, formation of a network structure of PEG via the inclusion complexation of γ-CyD with two PEG chains is probably important for the inhibitory effects on DNA release. These findings indicate that the combination of γ-CyD and PEG has potential as a blood-storage tool for liquid biopsy.

Effects of γ-CyD and PEG on (A) membrane fluidity, (B) viscosity of the solution, and (C) FALT of liposomes. Proposed mechanism for (D) cell aggregation and the subsequent DNA release and (E) the inhibition by γ-CyD/PEG. (F) Phospholipid concentration in the supernatant and (G) precipitates of liposome after centrifugation for 600 s (n = 6). *p < 0.05, compared with DMPC-liposome alone. p < 0.05, compared with + γ-CyD. p < 0.05, compared with + PEG.

Fumika Nakagawa, Yoshihisa Shimamura, Yusuke Suzuki, Toru Taharabaru, Hiroshi Itou, Yoichiro Iwase, Risako Onodera, Taishi Higashi, Keiichi Motoyama (2022) Application of cyclodextrin/polyethylene glycol in blood-storage tools for liquid biopsy. Materials Letters 324, 132660.

Leave a Reply

This site uses Akismet to reduce spam. Learn how your comment data is processed.