In recent years, liquid biopsy has garnered considerable attention as a valuable diagnostic technology for cancer. An analysis target of liquid biopsy is cell-free DNA; however, contamination with wild-type DNA released from injured leukocytes leads to low test accuracy. Thus, there is a need for blood-storage tools that inhibit the release of wild-type DNA from leukocytes. In the present study, we demonstrated that γ-cyclodextrin (γ-CyD) and polyethylene glycol (PEG) synergistically inhibit DNA release from cells by inhibiting cell aggregation. In addition, formation of a network structure of PEG via the inclusion complexation of γ-CyD with two PEG chains is probably important for the inhibitory effects on DNA release. These findings indicate that the combination of γ-CyD and PEG has potential as a blood-storage tool for liquid biopsy.
Fumika Nakagawa, Yoshihisa Shimamura, Yusuke Suzuki, Toru Taharabaru, Hiroshi Itou, Yoichiro Iwase, Risako Onodera, Taishi Higashi, Keiichi Motoyama (2022) Application of cyclodextrin/polyethylene glycol in blood-storage tools for liquid biopsy. Materials Letters 324, 132660.