Posted by In this study, the chiral separation of 38 analytes was investigated with capillary electrophoresis and the enantiomeric selectivity was compared utilizing -d-CD and -l-CD as CE pseudophases. Additionally, the parameters affecting the CE separation, including pH of the run buffer, capillary voltage, and ionic strength of the buffer, were examined. For the first time, the opposite enantioselectivity of the -l-CD was utilized to facilitate challenges in the quantitation of chiral impurities in pharmaceutical compounds.
Capillary electropherograms showing the enantiomeric separation of the stereoisomers of 2-(methylamino)-1-phenylpropan-1-ol using a segment of solution with concentration of 15 mM of A) 𝛽-d-CD and B) 𝛽-l-CD as the chiral selector in a run buffer, consisting of 200 mM sodium dihydrogen phosphate with pH of 2.2. The separation voltage was 25 kV.
Saba Aslani, Ryan Jacob Burk, Yong Wu, Daniel W. Armstrong (2025) Mirror-Image β-l-Cyclodextrin as a Chiral Pseudophase in Capillary Electrophoresis. Electrophoresis 47, 22-31. https://doi.org/10.1002/elps.70061