Posted by Surface Plasmon Resonance (SPR) technology, as a powerful analytical tool, plays a crucial role in the preparation, performance evaluation, and biomedical applications of nanoparticles due to its real-time, label-free, and highly sensitive detection capabilities. In the nanoparticle preparation process, SPR technology can monitor synthesis reactions and surface modifications in real-time, optimizing preparation techniques and conditions. SPR enables precise measurement of interactions between nanoparticles and biomolecules, including binding affinities and kinetic parameters, thereby assessing nanoparticle performance. In biomedical applications, SPR technology is extensively used in the study of drug delivery systems, biomarker detection for disease diagnosis, and nanoparticle-biomolecule interactions [1]. This paper reviews the latest advancements in SPR technology for nanoparticle preparation, performance evaluation, and biomedical applications, discussing its advantages and challenges in biomedical applications, and forecasting future development directions.
The following CD-related example is mentioned: to rapidly discover effective inhibitors of melittin, researchers utilized a system of self-assembling nanoparticles (SNPs) formed by cyclodextrin polymers and adamantane derivatives. They immobilized biotinylated melittin on a chip surface containing avidin and rapidly screened eight self-assembling nanoparticles from a candidate drug library that could bind and neutralize the toxicity of melittin using SPR technology [2]. This step not only expedited the discovery process of inhibitors but also enhanced the precision of the screening process.
References:
[1] Zhang J, Liu B, Chen H, Zhang L, Jiang X. Application and Method of Surface Plasmon Resonance Technology in the Preparation and Characterization of Biomedical Nanoparticle Materials. Int J Nanomedicine. 2024;19:7049-7069. https://doi.org/10.2147/IJN.S468695
[2] Xu Y, Deng M, Zhang H, et al. Selection of affinity reagents to neutralize the hemolytic toxicity of melittin based on a self-assembled nanoparticle library. ACS Appl Mater Interfaces. 2020;12(14):16040–16049. https://doi.org/10.1021/acsami.0c00303