Dimethyl-alpha-CD (DMACD) was found to enhance sperm fertilization ability by removing phospholipids. It was concluded that in addition to the cholesterol acceptor albumin and methyl beta-CD (MBCD) also DMACD, not interacting with cholesterol, can induce capacitation. BSA, MBCD and DMACD increased sperm membrane fluidity, rearrangement of lipid raft and the ecrosome reaction. These findings support that phospholipid extraction is a novel trigger of capacitation.
Nakao S, Ito K, Sakoh K, Takemoto K, Watanabe H, Kondoh G, Irie T, Nakagata N, Takeo T. Dimethyl-α-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm. Biol Reprod. 2023 Feb 1:ioad013. https://doi.org/10.1093/biolre/ioad013