Beta cyclodextrin (β-CD) was used for cholesterol removal from shrimp lipid using ethyl acetate and water as solvents. The cholesterol incorporating β-CD complex (β-CD–CL) was collected and β-CD incorporated with cholesterol (β-CD–CL) was further processed using ethanol with the aid of ultrasound to obtain cholesterol free β-CD (R-β-CD). Recycled β-CD (R-β-CD) was compared with pure β-CD (P-β-CD) for the reusability of cholesterol removal from shrimp lipid. R-β-CD showed 94% cholesterol removal, while 95% was achieved for P-β-CD. Differential Scanning Calorimetry (DSC) showed a slight decrease in the melting point of R-β-CD. Nevertheless, FTIR and NMR results revealed that functional groups and the proton spectrum of R-β-CD was negligibly altered. Fatty acid contents of treated oil were slightly higher when treated with R-β-CD than those of the lipid subjected to P-β-CD treatment. Reusability of β-CD could be achieved as confirmed by the maintained capacity in cholesterol removal and unaltered structure.
Navaneethan Raju, Avtar Singh and Soottawat Benjakul (2021) Recovery, reusability and stability studies of beta cyclodextrin used for cholesterol removal from shrimp lipid. RSC Adv., 11, 23113-23121. DOI: 10.1039/D1RA03282H