After oral exposure, β-CD passes through the digestive tract, where it may interact with the gut microbiota in the luminal environment. The gut microbiota is composed of different types of bacteria, viruses and fungi, and comprises 10-fold more cells than the human body.
In this study, the effects of oral exposure to β-CD on serum metabolites and gut microbiota were investigated in mice. Body weight or coefficients of organs were not changed after 14 days repeated oral administration. β-CD did not significantly affect the α-diversity indexes, but disturbed the structure of the gut microbiota according to principal component analysis (PCA).
After taxonomic assignment, 1 in 27 phyla, 2 in 48 classes, 3 in 107 orders, 6 in 192 families, and 8 in 332 genera were significantly different between control and β-CD treated groups. The serum metabolites were significantly changed after β-CD treatment. A total of 112 differential metabolites (89 downregulated and 23 upregulated) were identified.
The regulated genera of Ohtaekwangia, Paenibacillus, uncultured Candidatus Saccharibacteria bacterium, and Erysipelatoclostridium were closely related to many serum differential metabolites according to Pearson correlation analysis. The metabolic pathway of ABC transporters, pyrimidine metabolism, purine
metabolism, glucagon signaling pathway, and insulin signaling pathway were enriched by KEGG pathway analysis. This study revealed the effects of β-CD on gut microbiota, serum metabolites and their correlation, which should be considered before application.
Lv S, Zhang X, Feng Y, Jiang Q, Niu C, Yang Y and Wang X (2021) Gut Microbiota Combined With Metabolomics Reveals the Repeated Dose Oral Toxicity of β-Cyclodextrin in Mice.
Front. Pharmacol. 11:574607. https://www.frontiersin.org/article/10.3389/fphar.2020.574607