A novel water-soluble β-cyclodextrin branched polymer covalently integrating a fluorescein moiety and a nitric oxide (NO) photodonor within its macromolecular skeleton was prepared and studied in cooperation between University of Catania, University of Gothenburg and CycloLab.
Photoexcitation with visible light induces the parallel activation of the two chromophores, which results in the green fluorescence emission suitable for imaging accompanied by release of NO radical for therapy. This polymer internalizes in squamous carcinoma cancer cells (A431) in vitro, visualized by fluorescence microscopy, and induces cell mortality as a result of the NO photo-decaging. Furthermore, this new material is able to entrap an additional water-insoluble photoactive guest (porphyrin) and deliver it into the cells, without precluding the photochemical properties.
Milo Malanga, Mimimorena Seggio, Vladimir Kirejev, Aurore Fraix, Ivana Di Bari, Eva Fenyvesi, Marica B. Ericson and Salvatore Sortino: A phototherapeutic fluorescent β-cyclodextrin branched polymer delivering nitric oxide. Biomater. Sci., 7, 2272 (2019) DOI: 10.1039/c9bm00395a