Phase solubility indicated the 1:1 stoichiometry. FTIR, DSC, PXRD, and SEM analysis confirmed the formation of inclusion complex. Moreover, 1H NMR studies illustrated that the possible inclusion mode was through A-ring of the CHR which was further confirmed by molecular modelling. In addition, CHR/SBE-β-CD complex exhibited ∼108 times higher solubility and faster dissolution compared to CHR. The pH stability profile exhibited the protective effect of SBE-β-CD on the CHR at simulated pH. Also, in vitro cytotoxicity assay revealed an appreciable improvement in the anticancer activity of complex compared to free CHR, on MCF-7 and HeLa cell lines validating its superiority as a carrier.
 Abhijeet Kulkarni, Veena Belgamwar (2020) Inclusion complex of chrysin with sulfobutyl ether-β-cyclodextrin (Captisol®):Preparation, characterization, molecular modeling and in vitro anticancer activity. Journal of Molecular Structure 1128. DOI: 10.1016/j.molstruc.2016.09.025